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Vitamin D Testing Market Report


Vitamin D Testing Market by Product (25-Hydroxy Vitamin D Test and 1,25-Dihydroxy Vitamin D Test), Indication (Osteoporosis, Rickets, Thyroid Disorders, Vitamin D Deficiency, and Others), Technology (Radioimmunoassay, ELISA, HPLC, LC-MS, and Others), Patient (Adult and Pediatric), Test Location (Point-of-Care and Others), End-use (Hospitals, Diagnostic Laboratories, Homecare, and Others), and Region (North America, Europe, Asia-Pacific, and LAMEA): Opportunity Analysis and Industry Forecast, 2023-2032


Pages: 382

Sep 2023

Vitamin D Testing Overview

D testing is a medical procedure used to measure vitamin D levels in a person’s blood. Vitamin D, a significant nutrient, is noteworthy in maintaining bone health, supporting the immune system, and promoting overall well-being. The test focuses on the two primary shapes of vitamin D in the blood: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). The 25-hydroxyvitamin D test, known as the 25(OH)D test, is the most common method to assess vitamin D levels. It gives an overall picture of a person’s vitamin D status by measuring both forms. Healthcare providers ordinarily order this test for different reasons, such as diagnosing deficiency or insufficiency, checking supplementation, evaluating certain medical conditions related to vitamin D, or evaluating health in specific populations at hazard. The test results are detailed in nanograms per milliliter (ng/mL) or nanomoles per liter (nmol/L), with the optimal range falling between 20-50 ng/mL (50-125 nmol/L) for most individuals.

Global Vitamin D Testing Market Analysis

The global vitamin D testing market size was $850.67 million in 2022 and is predicted to grow with a CAGR of 8.3%, by generating a revenue of $1,789.67 million by 2032.

COVID-19 Impact on Global Vitamin D Testing Market

The COVID-19 pandemic had several implications for the vitamin D testing market. One notable effect was the increased demand for vitamin D testing as there was growing awareness of its potential role in immune health and COVID-19 outcomes. However, the shift in healthcare priorities towards managing the pandemic resulted in resources being redirected, potentially affecting the overall demand for routine testing, including vitamin D testing. To minimize exposure risks, telemedicine and home testing options gained popularity, allowing individuals to test their vitamin D levels from the comfort of their homes. The disruption caused by the pandemic also influenced production and supply chains, leading to potential fluctuations in the availability and pricing of testing kits. Additionally, extensive research into COVID-19 and its potential treatments may have further influenced the understanding of vitamin D's role in immunity and impacted the demand for testing.

Increasing Awareness of Vitamin D Deficiency to Drive the Market Growth

The vitamin D testing market is influenced by various factors that drive its growth. One significant factor is the increasing awareness of the importance of vitamin D in maintaining overall health, leading to more people and healthcare professionals seeking testing services. Additionally, the prevalence of vitamin D deficiency and related diseases in different regions and population groups has prompted a rise in testing to assess and address deficiencies. Furthermore, the rising healthcare expenditure and government initiatives promoting preventive healthcare have encouraged the inclusion of vitamin D testing in routine health check-ups, which is predicted to boost the growth of the global market during the forecast period.

High Cost of Testing to Restrain the Market Growth

The vitamin D testing market faces several restraining factors. The cost of testing might deter some individuals, especially in regions without adequate health insurance coverage. Emerging alternative diagnostic methods for assessing vitamin D levels could compete with traditional laboratory-based tests, impacting market demand. Shifting healthcare priorities and inconsistent guidelines from medical organizations may also contribute to reduced investment and promotion of vitamin D testing services. Seasonal variations in vitamin D levels and the popularity of over-the-counter supplements may further decrease the perceived need for testing.

Increasing Aging Population to Drive Excellent Opportunities

The vitamin D testing market presents significant opportunities due to several factors. The aging population’s higher risk of deficiency necessitates frequent testing to monitor and address imbalances. Furthermore, as preventive healthcare gains popularity, vitamin D testing plays a crucial role in early detection and proactive management of deficiencies. Besides, advancements in testing methodologies have made vitamin D testing more accurate and accessible. Additionally, the growing market for vitamin D supplements and fortified foods has increased the need for testing to ensure proper dosing and efficacy. Government support, reimbursement policies, and ongoing research & development further drive market growth. Moreover, the advent of point-of-care testing devices provides opportunities to expand the market's reach and improve patient outcomes, making vitamin D testing a promising sector for investment and innovation.

Global Vitamin D Testing Market Share, by Product, 2022

The 25-hydroxy vitamin D test sub-segment accounted for the highest market share in 2022. This is mainly because 25-hydroxy is the primary circulating form of vitamin D in the bloodstream, providing measurement stability and reliability. Unlike the active form of vitamin D, 1,25-Dihydroxy vitamin D, it has a longer half-life and remains more stable. This stability makes it the preferred choice for assessing an individual's vitamin D status. Moreover, 25-Hydroxy vitamin D levels are widely recognized as the best indicator of vitamin D sufficiency or deficiency, driving its clinical relevance. The prevalence of vitamin D deficiency worldwide has also increased the demand for such 25-hydroxy vitamin D tests.

Global Vitamin D Testing Market Share, by Indication, 2022

The osteoporosis sub-segment accounted for the highest market share in 2022. Osteoporosis has influenced the vitamin D testing market due to its close relationship with vitamin D deficiency. Osteoporosis is characterized by low bone density and an extended hazard of fractures. In contrast, vitamin D is fundamental to bone health by aiding calcium absorption. Subsequently, vitamin D testing has become pivotal in identifying people at risk of osteoporosis or related conditions. As the prevalence of osteoporosis rises, especially among aging populaces, the demand for vitamin D testing has expanded.

Global Vitamin D Testing Market Share, by Technology, 2022

The LC-MS sub-segment accounted for the highest market share in 2022. LC-MS (Liquid Chromatography-Mass Spectrometry) has become the dominant method in the vitamin D testing market due to its numerous advantages. This technique offers high accuracy and sensitivity in measuring vitamin D levels, allowing for the precise detection of different forms and metabolites. Its specificity ensures minimal interference from other compounds, leading to reliable and reproducible results, crucial for medical diagnosis. Moreover, LC-MS has a wide dynamic range, enabling measurement in severe deficiencies and high-dose supplement cases. Standardization across laboratories further enhances its appeal, and the growing awareness of vitamin D deficiency has fueled demand for accurate testing, solidifying LC-MS as a preferred choice.

Global Vitamin D Testing Market Share, by Patient, 2022

The adult sub-segment accounted for the highest market share in 2022. This is mainly because there has been a significant increase in awareness regarding the significance of vitamin D for overall health and its role in preventing different chronic diseases. This has prompted more adults to seek vitamin D testing to assess their levels and take proactive measures to maintain optimal health. Additionally, the aging population has contributed to the prominence of adults in this market, as older individuals are more susceptible to vitamin D deficiency due to age-related factors. Moreover, the prevalence of chronic diseases in adults linked to vitamin D deficiency has led healthcare providers to prioritize testing in this demographic.

Global Vitamin D Testing Market Share, by Test Location, 2022

The point-of-care sub-segment accounted for the highest market share in 2022. The  Dominant growth of this sub-segment is mainly because the convenience and accessibility offered by POC testing allows healthcare professionals to conduct tests and receive immediate results at the patient's location, eliminating the need for separate laboratory visits. The rapid turnaround time of POC tests enables timely intervention and treatment for patients with vitamin D deficiencies, particularly in cases requiring urgent attention. Moreover, the cost-effectiveness of POC testing has made it an attractive option for patients and healthcare facilities. Technological advancements in portable, user-friendly POC devices are further facilitating their adoption. Moreover, the growing awareness about the importance of vitamin D in maintaining overall health also contributed to the higher demand for testing, and POC testing is well-suited to meet this demand efficiently.

Global Vitamin D Testing Market Share, by End-use, 2022

The diagnostic laboratories sub-segment accounted for the highest market share in 2022.  The established expertise and accreditations of diagnostic laboratories in conducting various medical tests, including vitamin D testing, have built trust among patients and healthcare providers. The widespread accessibility and convenience of diagnostic laboratories with multiple branches in different regions make it easy for patients and healthcare providers to access vitamin D testing services. Moreover, these laboratories often offer comprehensive test panels, bundling vitamin D testing with routine check-ups or specialized assessments, attracting more customers and encouraging healthcare providers to prefer their services as one-stop solutions. The integration with healthcare providers, clinics, and hospitals, coupled with stringent quality assurance processes, assist strengthens their market dominance.

Global Vitamin D Testing Market Share, by Region, 2022

The North America vitamin D testing market generated the highest revenue in 2022. This is mainly because the North America region boasts an advanced healthcare infrastructure with well-equipped laboratories and skilled medical professionals, ensuring efficient and accurate testing services. Besides, the high prevalence of vitamin D deficiency among people of the region due to limited sunlight exposure and lifestyle choices, drives the demand for vitamin D testing. Furthermore, expanding awareness of the health benefits of vitamin D has driven a more significant number of people looking for such tests to maintain optimal health. The region's culture of regular health screening and preventive healthcare practices also contributes to the popularity of vitamin D testing.

Competitive Scenario in the Global Vitamin D Testing Market

Investment and agreement are common strategies followed by the major market players. One of the leading market players in the industry is DiaSorin S.p.A. In February 2021, DiaSorin used the LIAISON analyzer family to use chemiluminescence for the quantitative measurement and analysis of 25-hydroxyvitamin D and other hydroxylated vitamin D metabolites in human serum. Advanced techniques such as immunoassay (CLIA) were utilized.

Some of the leading vitamin D testing market players are Abbott, Danaher, F. Hoffmann-La Roche Ltd, Siemens Healthineers AG, Quest Diagnostics Incorporated., BIOMÉRIEUX, DiaSorin S.p.A., Thermo Fisher Scientific Inc., Beckman Coulter, Inc. (BD), and Tosoh Bioscience, Inc.



Historical Market Estimations


Base Year for Market Estimation


Forecast Timeline for Market Projection


Geographical Scope

North America, Europe, Asia-Pacific, and LAMEA

Segmentation by Product

  • 25-Hydroxy Vitamin D Test
  • 1,25-Dihydroxy Vitamin D Test

Segmentation by Indication

  • Osteoporosis
  • Rickets
  • Thyroid Disorders
  • Vitamin D Deficiency
  • Others


Segmentation by Technology



  • Radioimmunoassay
  • HPLC
  • LC-MS
  • Others

Segmentation by Patient


  • Adult
  • Pediatric

Segmentation by Test Location


  • Point-of-Care
  • Others

Segmentation by End-use


  • Hospitals
  • Diagnostic Laboratories
  • Homecare
  • Others

Key Companies Profiled

  • Abbott
  • Danaher
  • F. Hoffmann-La Roche Ltd
  • Siemens Healthineers AG
  • Quest Diagnostics Incorporated.
  • DiaSorin S.p.A.
  • Thermo Fisher Scientific Inc.
  • Beckman Coulter, Inc. (BD)
  • Tosoh Bioscience, Inc.

Frequently Asked Questions

A. The size of the global vitamin D testing market was over $850.67 million in 2022 and is projected to reach $1,789.67 million by 2032.

A. Beckman Coulter, Inc. (BD) and Tosoh Bioscience, Inc. are some of the key players in the global vitamin D testing market.

A. The North America region possesses great investment opportunities for investors to witness the most promising growth in the future.

A. Agreement and investment are the two key strategies opted by the operating companies in this market.

A. Quest Diagnostics Incorporated., BIOMÉRIEUX, DiaSorin S.p.A., and Thermo Fisher Scientific Inc. are the companies investing more on R&D activities for developing new products and technologies.

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